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  • Sorbitol can be produced not only chemically but also biotechnologically.

Sorbitol can be produced not only chemically but also biotechnologically.

Applied biochemistry and biotechnology (2004-08-12)
Rainer Jonas, Mauricio M Silveira
ABSTRACT

Sorbitol, a polyol found in many fruits, is attracting increasing industrial interest as a sweetener, humectant, texturizer, and softener. It is principally produced by chemical means. The bacterium Zymomonas mobilis is able to produce sorbitol together with gluconic acid from fructose and glucose, respectively. This is possible in a one-step reaction via the enzyme glucose-fructose oxidoreductase, so far only known from Z. mobilis. The possibilities for the production of sorbitol by Z. mobilis are discussed also under the aspect of an industrial process and compared with the current chemical as well as other microbiologic processes. The production process by Z. mobilis shows economic possibilities for certain countries, such as Brazil, considering only the products sorbitol and ethanol as an important byproduct. For the other byproduct, gluconic acid, further studies for its partial substitution must be conducted.

MATERIALS
Product Number
Brand
Product Description

Supelco
Sorbitol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
D-Sorbitol, 99% (GC)
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D-Sorbitol, FCC, FG
Sigma-Aldrich
D-Sorbitol, liquid, tested according to Ph. Eur.
Sigma-Aldrich
D-Sorbitol, BioUltra, ≥99.0% (HPLC)
Sigma-Aldrich
D-Sorbitol, ≥98% (GC)
Sigma-Aldrich
D-Sorbitol, ≥98% (GC), Molecular Biology
Sigma-Aldrich
D-Sorbitol, ≥98% (GC), BioXtra
Sigma-Aldrich
D-Sorbitol, ≥98% (GC), BioReagent, suitable for cell culture, suitable for plant cell culture
Sorbitol, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Sorbitol F solution, 70 wt. % in H2O, Contains mainly D-sorbitol with lesser amounts of other hydrogenated oligosaccharides
Sigma-Aldrich
D-Sorbitol, Vetec, reagent grade, 97%
USP
Sorbitol, United States Pharmacopeia (USP) Reference Standard