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O-GlcNAc signaling in cancer metabolism and epigenetics.

Cancer letters (2014-04-29)
Jay Prakash Singh, Kaisi Zhang, Jing Wu, Xiaoyong Yang
ABSTRACT

The covalent attachment of β-D-N-acetylglucosamine monosaccharides (O-GlcNAc) to serine/threonine residues of nuclear and cytoplasmic proteins is a major regulatory mechanism in cell physiology. Aberrant O-GlcNAc modification of signaling proteins, metabolic enzymes, and transcriptional and epigenetic regulators has been implicated in cancer. Relentless growth of cancer cells requires metabolic reprogramming that is intertwined with changes in the epigenetic landscape. This review highlights the emerging role of protein O-GlcNAcylation at the interface of cancer metabolism and epigenetics.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
N-Acetyl-D-glucosamine, ≥95% (HPLC)
SAFC
L-Threonine
Sigma-Aldrich
N-Acetyl-D-glucosamine, suitable for cell culture, BioReagent
Sigma-Aldrich
N-Acetyl-D-glucosamine, ≥99% (HPLC)
Sigma-Aldrich
L-Threonine, reagent grade, ≥98% (HPLC)
L-Threonine, European Pharmacopoeia (EP) Reference Standard
Supelco
N-Acetylglucosamine, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
L-Threonine, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
L-Threonine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
L-Threonine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 99.0-101.0%
Sigma-Aldrich
L-Threonine, BioXtra, ≥99.5% (NT)
Serine, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Phosphorylase b from rabbit muscle, For use as a marker in SDS-PAGE
Sigma-Aldrich
Phosphorylase b from rabbit muscle, lyophilized powder, ≥20 units/mg protein, 2× crystallization
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L-Serine, ReagentPlus®, ≥99% (HPLC)
Supelco
L-Serine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
L-Serine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Supelco
L-Serine, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L-Serine, BioUltra, ≥99.5% (NT)