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  • Regulation of auxin, abscisic acid and salicylic acid levels by ascorbate application under heat stress in sensitive and tolerant maize leaves.

Regulation of auxin, abscisic acid and salicylic acid levels by ascorbate application under heat stress in sensitive and tolerant maize leaves.

Acta biologica Hungarica (2014-12-06)
Burcu Seckin Dinler, Emel Demir, Yasemin Ozdener Kompe
ABSTRACT

In the present study, the effect of ascorbic acid (5 mM) on some physiological parameters and three hormones (auxin, abscisic acid, salicylic acid) was determined under heat stress (40 °C) in maize tolerant cv. (MAY 69) and sensitive cv. SHEMAL (SH) at 0 h, 4 h and 8 h. Heat stress reduced total chlorophyll content (CHL), relative water content (RWC) and stomatal conductance (gs) in SH but did not lead to changes in MAY 69 at 4 h and 8 h. However, pretreatment with ascorbic acid increased (CHL), (RWC) and (gs) in SH under heat stress while it reduced MDA content significantly in both cv. We also observed that heat stress led to a reduction in SA level but increased ABA and IAA levels in SH, whereas it increased SA and IAA levels but did not change ABA level in MAY 69 at 4 h. Furthermore, in SH, ASC application under heat stress increased SA level and decreased IAA and ABA levels at 4 h, but it had no effect on SA and ABA at 8 h.

MATERIALS
Product Number
Brand
Product Description

Supelco
Water, suitable for HPCE, suitable for luminescence, suitable for UV/Vis spectroscopy
Sigma-Aldrich
Water, ACS reagent
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Water, Molecular Biology, sterile filtered
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L-Ascorbic acid, powder, suitable for cell culture, γ-irradiated
Supelco
L-Ascorbic acid, analytical standard
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Water, sterile-filtered, BioReagent, suitable for cell culture
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(+)-Sodium L-ascorbate, powder, BioReagent, suitable for cell culture
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Salicylic acid, suitable for plant cell culture
Pure Water Density Standard, UKAS ISO/IEC17025 and ISO Guide 34 certified, density: 0.9982 g/mL at 20 °C, density: 0.9970 g/mL at 25 °C
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(+)-Sodium L-ascorbate, crystalline, ≥98%
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(+)-Sodium L-ascorbate, BioXtra, ≥99.0% (NT)
Supelco
Density Standard 998 kg/m3, H&D Fitzgerald Ltd. Quality
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Water, Nuclease-Free Water, for Molecular Biology
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Water, for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture
USP
Salicylic acid, United States Pharmacopeia (USP) Reference Standard
Sodium ascorbate, European Pharmacopoeia (EP) Reference Standard
Supelco
Sodium ascorbate, Pharmaceutical Secondary Standard; Certified Reference Material
Salicylic acid, European Pharmacopoeia (EP) Reference Standard
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Water, BioPerformance Certified
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Water, PCR Reagent
Supelco
Salicylic acid, Pharmaceutical Secondary Standard; Certified Reference Material
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Salicylic acid, puriss. p.a., ≥99.0% (T)
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Salicylic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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Salicylic acid, ACS reagent, ≥99.0%
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Salicylic acid, ReagentPlus®, ≥99%
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Salicylic acid, BioXtra, ≥99.0%
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L-Ascorbic acid, ACS reagent, ≥99%
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L-Ascorbic acid, reagent grade, crystalline
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L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%
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L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)