- A novel stable isotope labelling assisted workflow for improved untargeted LC-HRMS based metabolomics research.
A novel stable isotope labelling assisted workflow for improved untargeted LC-HRMS based metabolomics research.
Metabolomics : Official journal of the Metabolomic Society (2014-07-25)
Christoph Bueschl, Bernhard Kluger, Marc Lemmens, Gerhard Adam, Gerlinde Wiesenberger, Valentina Maschietto, Adriano Marocco, Joseph Strauss, Stephan Bödi, Gerhard G Thallinger, Rudolf Krska, Rainer Schuhmacher
PMID25057268
ABSTRACT
Many untargeted LC-ESI-HRMS based metabolomics studies are still hampered by the large proportion of non-biological sample derived signals included in the generated raw data. Here, a novel, powerful stable isotope labelling (SIL)-based metabolomics workflow is presented, which facilitates global metabolome extraction, improved metabolite annotation and metabolome wide internal standardisation (IS). The general concept is exemplified with two different cultivation variants, (1) co-cultivation of the plant pathogenic fungi
MATERIALS
Product Number
Brand
Product Description
Supelco
Residual Solvent - Acetonitrile(solution in DMSO), Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
Supelco
Citric acid, Anhydrous, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Potassium phosphate monobasic, meets analytical specification of Ph. Eur., NF, E340, anhydrous, 98-100.5% (calc. to the dried substance)
Sigma-Aldrich
Potassium phosphate monobasic, buffer substance, anhydrous, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99.5-100.5%