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  • Endothelial cell-initiated extravasation of cancer cells visualized in zebrafish.

Endothelial cell-initiated extravasation of cancer cells visualized in zebrafish.

PeerJ (2015-01-01)
Masamitsu Kanada, Jinyan Zhang, Libo Yan, Takashi Sakurai, Susumu Terakawa
PMID25551022
ABSTRACT

The extravasation of cancer cells, a key step for distant metastasis, is thought to be initiated by disruption of the endothelial barrier by malignant cancer cells. An endothelial covering-type extravasation of cancer cells in addition to conventional cancer cell invasion-type extravasation was dynamically visualized in a zebrafish hematogenous metastasis model. The inhibition of VEGF-signaling impaired the invasion-type extravasation via inhibition of cancer cell polarization and motility. Paradoxically, the anti-angiogenic treatment showed the promotion, rather than the inhibition, of the endothelial covering-type extravasation of cancer cells, with structural changes in the endothelial walls. These findings may be a set of clues to the full understanding of the metastatic process as well as the metastatic acceleration by anti-angiogenic reagents observed in preclinical studies.

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