Herbal medicine Guan Chang Fu Fang enhances 5-fluorouracil cytotoxicity and affects drug-associated genes in human colorectal carcinoma cells.

Herbal medicine Guan Chang Fu Fang enhances 5-fluorouracil cytotoxicity and affects drug-associated genes in human colorectal carcinoma cells.

Oncology letters (2015-01-27)

Chen Yu, Shen-Lin Liu, Ming-Hao Qi, Xi Zou, Jian Wu, Jing Zhang

PMID25621039

ABSTRACT

Guan Chang Fu Fang (GCFF) is a natural compound, which is extracted from three medicinal plants,

MATERIALS

Product Number

Brand

Product Description

606766

Sigma-Aldrich

Acetic acid-¹²C₂, 99.9 atom % ¹²C

PHR1070

Supelco

Dehydrated Alcohol, Pharmaceutical Secondary Standard; Certified Reference Material

644374

Sigma-Aldrich

Methanol, purification grade, 99.8%

322415

Sigma-Aldrich

Methanol, anhydrous, 99.8%

45726

Sigma-Aldrich

Acetic acid, suitable for luminescence, BioUltra, ≥99.5% (GC)

W200611

Sigma-Aldrich

Acetic acid, ≥99.5%, FCC, FG

W200603

Sigma-Aldrich

Acetic acid, natural, ≥99.5%, FG

F8423

Sigma-Aldrich

Fluorouracil, meets USP testing specifications

2858

Sigma-Aldrich

Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)

G5251

Sigma-Aldrich

DL-Glyceraldehyde 3-phosphate solution, 45-55 mg/mL in H₂O

717975

Sigma-Aldrich

Methanol, suitable for NMR (reference standard)

27225

Sigma-Aldrich

Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.8-100.5%

F6627

Sigma-Aldrich

5-Fluorouracil, ≥99% (HPLC), powder

179957

Sigma-Aldrich

Methanol, Laboratory Reagent, ≥99.6%

03738

Supelco

5-Fluorouracil, analytical standard

82762

Supelco

Methanol, analytical standard

F0250000

Fluorouracil, European Pharmacopoeia (EP) Reference Standard

71251

Supelco

Acetic acid, analytical standard

48075

Supelco

Ethanol solution, certified reference material, 2000 μg/mL in methanol

E2385

Supelco

Ethanol standards 10% (v/v), 10 % (v/v) in H₂O, analytical standard

1279000

USP

Fluorouracil, United States Pharmacopeia (USP) Reference Standard

PHR1372

Supelco

Methanol, Pharmaceutical Secondary Standard; Certified Reference Material

676780

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

695092

Sigma-Aldrich

Acetic acid, glacial, ACS reagent, ≥99.7%

154903

Sigma-Aldrich

Methanol, ACS spectrophotometric grade, ≥99.9%

E7148

Sigma-Aldrich

Ethyl alcohol, Pure 190 proof, for molecular biology

179337

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

A6283

Sigma-Aldrich

Acetic acid, glacial, ReagentPlus^®, ≥99%

27221

Sigma-Aldrich

Acetic acid, glacial, puriss., 99-100%

V900394

Sigma-Aldrich

5-Fluorouracil, Vetec^™, reagent grade, ≥99%