Merck
CN
  • Initial stem cell adhesion on porous silicon surface: molecular architecture of actin cytoskeleton and filopodial growth.

Initial stem cell adhesion on porous silicon surface: molecular architecture of actin cytoskeleton and filopodial growth.

Nanoscale research letters (2014-11-12)
Pierre-Yves Collart-Dutilleul, Ivan Panayotov, Emilie Secret, Frédérique Cunin, Csilla Gergely, Frédéric Cuisinier, Marta Martin
PMID25386101
ABSTRACT

The way cells explore their surrounding extracellular matrix (ECM) during development and migration is mediated by lamellipodia at their leading edge, acting as an actual motor pulling the cell forward. Lamellipodia are the primary area within the cell of actin microfilaments (filopodia) formation. In this work, we report on the use of porous silicon (pSi) scaffolds to mimic the ECM of mesenchymal stem cells from the dental pulp (DPSC) and breast cancer (MCF-7) cells. Our atomic force microscopy (AFM), fluorescence microscopy, and scanning electron microscopy (SEM) results show that pSi promoted the appearance of lateral filopodia protruding from the DPSC cell body and not only in the lamellipodia area. The formation of elongated lateral actin filaments suggests that pores provided the necessary anchorage points for protrusion growth. Although MCF-7 cells displayed a lower presence of organized actin network on both pSi and nonporous silicon, pSi stimulated the formation of extended cell protrusions.

MATERIALS
Product Number
Brand
Product Description
702102
Sigma-Aldrich
Carbon, mesoporous
V900392
Sigma-Aldrich
D-(+)-Glucose, Vetec™, reagent grade, ≥99.5% (HPLC)
702110
Sigma-Aldrich
Carbon, mesoporous, hydrophilic pore surface
93067
Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX2, powder, from peat, multi-purpose activated charcoal, steam activated and acid washed
22874
Sigma-Aldrich
Activated Charcoal Norit®, Norit® PK 1-3, from peat, steam activated, granular
37771
Sigma-Aldrich
Activated Charcoal Norit®, Norit® GAC 1240W, from coal, for potable water processing, steam activated, granular
53663
Sigma-Aldrich
Activated Charcoal Norit®, Norit® SX ultra, from peat, corresponds U.S. Food chemicals codex (3rd Ed.), steam activated and acid washed, highly purified, powder
29204
Sigma-Aldrich
Activated Charcoal Norit®, Norit® RB3, for gas purification, steam activated, rod
29238
Supelco
Activated Charcoal Norit®, Norit® RBAA-3, rod
48075
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
G7651
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
G6257
Sigma-Aldrich
Glutaraldehyde solution, Grade II, 25% in H2O
G5882
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
G6403
Sigma-Aldrich
Glutaraldehyde solution, 50% in H2O, suitable for photographic applications
G7021
Sigma-Aldrich
D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
G5146
Sigma-Aldrich
D-(+)-Glucose, Hybri-Max™, powder, BioReagent, suitable for hybridoma
G7528
Sigma-Aldrich
D-(+)-Glucose, ≥99.5% (GC), BioXtra
2858
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
E2385
Supelco
Ethanol standards 10% (v/v), 10 % (v/v) in H2O, analytical standard
G6152
Sigma-Aldrich
D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)
47083-U
Supelco
Formaldehyde solution, stabilized with methanol, ~37 wt. % in H2O, certified reference material
F8775
Sigma-Aldrich
Formaldehyde solution, for molecular biology, 36.5-38% in H2O
G7776
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
G7526
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
G8270
Sigma-Aldrich
D-(+)-Glucose, ≥99.5% (GC)
G5767
Sigma-Aldrich
D-(+)-Glucose, ACS reagent
15512
Sigma-Aldrich
Formaldehyde solution, meets analytical specification of USP, ≥34.5 wt. %
49139
Sigma-Aldrich
D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
49152
Sigma-Aldrich
D-(+)-Glucose, tested according to Ph. Eur.
47608
Sigma-Aldrich
Formaldehyde solution, for molecular biology, BioReagent, ≥36.0% in H2O (T)