- A minimalist biosensor: Quantitation of cyclic di-GMP using the conformational change of a riboswitch aptamer.
A minimalist biosensor: Quantitation of cyclic di-GMP using the conformational change of a riboswitch aptamer.
RNA biology (2015-06-27)
Colleen A Kellenberger, Jade Sales-Lee, Yuchen Pan, Madalee M Gassaway, Amy E Herr, Ming C Hammond
PMID26114964
ABSTRACT
Cyclic di-GMP (c-di-GMP) is a second messenger that is important in regulating bacterial physiology and behavior, including motility and virulence. Many questions remain about the role and regulation of this signaling molecule, but current methods of detection are limited by either modest sensitivity or requirements for extensive sample purification. We have taken advantage of a natural, high affinity receptor of c-di-GMP, the Vc2 riboswitch aptamer, to develop a sensitive and rapid electrophoretic mobility shift assay (EMSA) for c-di-GMP quantitation that required minimal engineering of the RNA.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.8-100.5%
Sigma-Aldrich
Sodium hydroxide, anhydrous, free-flowing, Redi-Dri™, reagent grade, ≥98%, pellets
Sigma-Aldrich
Sodium hydroxide, puriss. p.a., ACS reagent, reag. Ph. Eur., K ≤0.02%, ≥98%, pellets
Sigma-Aldrich
Sodium hydroxide, puriss., meets analytical specification of Ph. Eur., BP, NF, E524, 98-100.5%, pellets