Merck
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  • Production of short-chain fatty acids from the biodegradation of wheat straw lignin by Aspergillus fumigatus.

Production of short-chain fatty acids from the biodegradation of wheat straw lignin by Aspergillus fumigatus.

Bioresource technology (2015-08-12)
Eduardo Baltierra-Trejo, Juan Manuel Sánchez-Yáñez, Otoniel Buenrostro-Delgado, Liliana Márquez-Benavides
ABSTRACT

The wheat straw lignin-rich fraction (WSLig-RF) can be used as a raw material for the production of metabolites for industrial use if ligninolytic mitosporic fungi are used for its biodegradation into aromatics and short-chain fatty acids (SCFAs, i.e., SCFA2-6). Although methods for the production of SCFA2-6 have been described previously, quantitative data of SCFA2-6 production have not been reported. The objectives of this study were to investigate the biodegradation of different concentrations of WSLig-RF by Aspergillus fumigatus and to identify whether SCFA2-6 production was dependent on the concentration of aromatics. A. fumigatus generated 2805mgL(-1) acetic acid when mixed with WSLig-RF at a concentration of 20gL(-1). Thus, aromatics are a substrate for the biosynthesis of SCFA2-6, and their production depends on the concentration of WSLig-RF aromatics.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
Sigma-Aldrich
Methanol solution, contains 0.50 % (v/v) triethylamine
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Acetic acid-12C2, 99.9 atom % 12C
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Acetic acid, natural, ≥99.5%, FG
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Hexanoic acid, ≥99%
Sigma-Aldrich
Hexanoic acid, ≥98%, FCC, FG
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Acetic acid, ≥99.5%, FCC, FG
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Propionic acid, BioReagent, suitable for insect cell culture, ~99%
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Isobutyric acid, 99%
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Propionic acid, natural, 99%, FG
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Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)
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Isovaleric acid, ≥99%, FCC, FG
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Isobutyric acid, natural, ≥99%, FCC, FG
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Butyric acid, ≥99%, FG
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Butyric acid, natural, ≥99%, FCC, FG
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Isobutyric acid, ≥99%, FCC, FG
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Methanol, anhydrous, 99.8%
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Valeric acid, ≥99%
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Propionic acid, ACS reagent, ≥99.5%
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Butyric acid, ≥99%
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Isovaleric acid, 99%
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Methanol, NMR reference standard
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Methanol-12C, 99.95 atom % 12C
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Propionic acid, puriss. p.a., ≥99.5% (GC)
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Methanol, purification grade, 99.8%
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Valeric acid, ≥99%, FCC, FG
Sigma-Aldrich
Isovaleric acid, natural, ≥98%, FG
Sigma-Aldrich
Propionic acid, ≥99.5%, FCC, FG