Skip to Content
Merck
CN

GC Analysis of a 38-Component FAME Mix on SP®-2560, 200 m Column

GC Analysis of a 38-Component FAME Mix on SP®-2560, 200 m Column suitable for GC

Materials

related product

Product No.
Description
Pricing

29688-U

SP®-2560 Capillary GC Column

L × I.D. 200 m × 0.25 mm, df 0.20 μm

CONDITIONS

column

SP-2560, 200 m x 0.25 mm I.D., 0.20 μm (29688-U)

oven

100 °C (13 min), 4 °C/min to 240 °C (30 min)

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

hydrogen, 27 cm/sec

injection

1.0 μL, 50:1 split

liner

4 mm I.D., split type, cup design

sample

Supelco 37-Component FAME Mix (47885-U) + C22:5n3 FAME

Description

Analysis Note

Identification and quantitation of individual fatty acid isomers in complex mixtures of isomers is desirable for both food and clinical scientists. This application requires the fatty acids to be converted to fatty acid methyl esters (FAMEs), followed by analysis using a long, efficient GC column with a stationary phase highly selective for polarizable analytes (those with double and/or triple C-C bonds). A 200 m SP-2560 is such a column.

Legal Information

SP is a registered trademark of Sigma-Aldrich Co. LLC

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS