GC Analysis of Bacterial Acid Methyl Esters (BAMEs) on Equity^™-1

column

Equity-1, 30 m × 0.25 mm I.D., 0.25 μm (28046-U)

oven

150 °C (4 min), 4 °C/min to 250°C (5 min)

inj. temp.

250 °C

detector

FID, 280 °C

carrier gas

helium, 20 cm/sec @ 150 °C

injection

1 μL, split 100:1

liner

split, cup design

sample

100 ng on-column of a 26 component BAME standard (47080-U)