GC Analysis of Conjugated Linoleic Acid (CLA) FAME Isomers on SP^®-2560

column

SP-2560, 200 meters x 0.25mm ID x 0.2um (29688-U)

oven

175 °C

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

hydrogen

injection

1μL, split 50:1

liner

4 mm I.D., split type, cup design

sample

4-component CLA FAME isomer mix, 0.5 mg/mL in methylene chloride