GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Soybean Oil on SLB®-35ms after SPE using Supelclean™ EZ-POP NP
Materials
SPE tube or plate
related product
Inlet Liner, Split/Splitless Type, Single Taper Fast FocusLiner™ Design (wool packed)
pkg of 1 eastandard
Polynuclear Aromatic Hydrocarbons Mix
certified reference material, 2000 μg/mL each component in benzene: dichloromethane (50:50), ampule of 1 mLCONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
soybean oil spiked with PAHs at 10 ng/g
SPE tube/cartridge
Supelclean EZ-POP NP, 2.5 g/12 mL (54341-U)
condition
10 mL acetone, dry under 10-15" Hg for 10 minutes
sample addition
0.5 mL weighed accurately onto cartridge
elution
15 mL acetonitrile
eluate post-treatment
concentrate to final volume of 0.5 mL at 40 °C under nitrogen
column
SLB-35ms, 30 m x 0.25 mm I.D., 0.25 μm (29804-U)
oven
60 °C (1 min), 20 °C/min to 340 °C (10 min)
inj. temp.
300 °C
detector
MSD-SIM (MS source at 250 °C, quads at 200°C)
MSD interface
330 °C
scan range
SIM
carrier gas
helium, 1 mL/min constant flow
liner
2 mm I.D. FocusLiner with taper
Description
Analysis Note
PAHs are ubiquitous in the environment, resulting from both natural and manmade sources of combustion. They can contaminate food crops through environmental exposure to particulates, automobile exhaust, etc. In the case of edible oils, additional PAHs can be introduced during actual production processes such as seed drying. In this application, EZ-POP NP SPE is used to extract PAHs from two popular edible oils, canola and soybean. The 16 PAHs designated in many US EPA methods were analyzed by GC/MS after extraction from spiked samples of oil.
Legal Information
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany
Supelclean is a trademark of Sigma-Aldrich Co. LLC