US EPA Method 608/8081/OLM04.2 PEST: GC Analysis of Organochlorine Pesticides on SLB®-5ms, 340 °C Final Temp.
CONDITIONS
column
SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)
oven
100 °C (2 min), 15 °C/min to 340 °C (5 min)
inj. temp.
250 °C
carrier gas
helium, 0.9 mL/min
injection
1μL, splitless (0.75 min)
liner
4 mm I.D., split/splitless type, wool packed single taper FocusLiner™ design
sample
200 ppm organochlorine pesticide standard mix (CRM46845) containing 22 analytes, diluted to 50 ppb with hexane
detector
μECD, 340 °C
Description
Analysis Note
The analysis of low levels of 20 organochlorine pesticides and 2 surrogate compounds by gas chromatography-electron capture detector (GC-ECD) is routinely performed by analysts in environmental laboratories. Methods require the analysis of each sample extract on two columns with differing selectivity for confirmation of identifications. The SLB-5ms/SLB-35ms pair is an effective column set because all 22 analytes are resolved between both columns using the same run conditions.
This applicatin shows the separation achieved on SLB-5ms. Peaks are well integrated and baselines are exceptionally stable considering the high final oven temperature (340 °C). Minimal baseline rise is detected, and the last analyte, decachlorobiphenyl, is eluted in less than 20 minutes.
Legal Information
FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany