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HILIC Analysis of Polar Vitamins on Ascentis® Express OH5

HILIC Analysis of Polar Vitamins on Ascentis® Express OH5 application for HPLC

Materials

related product

Product No.
Description
Pricing

53769-U

Ascentis® Express OH5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

CONDITIONS

column

Ascentis Express OH5 10 cm x 3.0 mm I.D. x 2.7 μm particles (53769-U)

mobile phase

100 mM ammonium formate:Acetonitrile (15:85, v/v), pH adjusted to 5.0 with formic acid

flow rate

0.5 mL/min

pressure

850 psi (58.6 bar)

column

35 °C

detector

UV, 276 nm

injection

2 μL

sample

100 ug/mL in 50:50 methanol:acetonitrile

Description

Analysis Note

This application demonstrates good retention and selectivity for polar vitamins on Ascentis® Express OH5 in HILIC mode.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS