HPLC (1D-Ag+-LC-ELSD) Analysis of Triacylglycerols in Gilthead Bream (Sparus aurata) on Nucleosil® SA following Extraction by Folch, Bligh & Dyer, and Maceration Methods
Materials
related product
ASTM® D6584 Triolein solution
certified reference material, 5000 μg/mL in pyridine, ampule of 1 × 2 mLCONDITIONS
column
Nucleosil SA (sulfonic acid), 25 cm x 4.6 mm I.D., 5 μm (50174-U)
mobile phase
[A] 0.5% butyronitrile (BCN) in hexane; [B] 5% butyronitrile (BCN) in hexane
gradient
0 to 100% B in 100 min
flow rate
0.8 mL/min
detector
ELSD
injection
10 μL
sample
fish tissue extracted by Folch, Bligh & Dyer, and maceration methods
Description
Analysis Note
Data provided by Marco Beccaria (1) and Luigi Mondello (1,2) of (1) Chromaleont s.r.l., c/o “Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy or (2) “Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy.
Triacylglycerols extracted from fish tissue were analyzed by an off-line combination of silver-ion liquid chromatography using a Nucleosil SA column and non-aqueous reversed-phase liquid chromatography (NARP) using an Ascentis Express C18 column. Shown here is the first dimension separation on the Nucleosil SA column. PN is the carbon number (CN) minus twice the double bond number (DBN).
Legal Information
Nucleosil is a registered trademark of Macherey-Nagel GmbH & Co. KG