HPLC Analysis of a Monoclonal Antibody Tryptic Digest on BIOshell A160 Peptide C18, 2.7 μm
CONDITIONS
sample preparation
MSQC4 monoclonal antibody, 0.5 g/L (final), digested with trypsin at high temperature per Perfinity Flash Digest protocol
column
BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)
mobile phase
[A] 98:2, (0.1% TFA in water):(0.09% TFA in acetonitrile); [B] 50:50, (0.1% TFA in water):(0.09% TFA in acetonitrile)
gradient
0 to 63%B in 60 min
flow rate
0.3 mL/min
pressure
4100 psi initial (total system) (283 bar)
detector
UV, 215 nm
injection
20 μL
Description
Analysis Note
Intact monoclonal antibody MSQC4 was digested with trypsin (iwthout denaturation or alkylation), and chromatographed on the BIOshell peptide column.
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