SPE (Solid Phase Extraction)
25 g corn meal in 100 mL acetonitrile:water (84:16)
Supel™ Tox AflaZea, 6 mL (55314-U)
2 mL
collect sample in 5 mL silanized glass vial
transfer 200 μL sample, add 300 μL TFA derivatization solution (70:20:10 water:TFA:acetic acid), heat in microreactor 20 min at 65 °C, cool, add 580 μL deionized water, vortex
Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U)
[A] water; [B] acetonitrile; (80:20, A:B)
0.350 mL/min
325 psi (22.4 bar)
35 °C
fluorescence, ex 360 nm, em 440 nm
20 μL
Aflatoxins are closely related compounds produced as secondary metablolites of fungi. They are found in grains and nuts like corn, wheat or peanuts. Shown here is a rapid, sensitive method to extract these compounds from corn using SPE cartridges specifically designed for aflatoxins, Supel Tox AflaZea (aflatoxin zearalenone), which removes interferences from grains, feeds, TMR samples, peanuts, peanut products and aqueous solutions. Analysis was via HPLC on Ascentis Express Fused-Core C18 colums.
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC