HPLC Analysis of Aminopterin and Related Compounds on Ascentis^® RP-Amide

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565352-U)

[A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)

1.0 mL/min

35 °C

UV, 280 nm

10 μL

This application demonstrates the suitability of the Ascentis RP-Amide for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis RP-Amide are presented.

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany