HPLC Analysis of B Vitamins on Ascentis® Express RP-Amide, Acetonitrile Mobile Phase
Materials
related product
Product No.
Description
Pricing
Thiamine HCl (Vitamin B1) solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Cyanocobalamin (Vitamin B12) solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 μm particles (50773-U)
mobile phase
[A] 0.02 M Ammonium Formate, pH = 3.0; [B] acetonitrile
gradient
0-50% B in 1 min. Hold at 50% B until 1.5 min.
flow rate
1.5 mL/min
pressure
1639 psi (113 bar)
column temp.
30 °C
detector
UV, 276 nm
injection
1 μL
sample
dissolved in 50/50 water/acetonitrile
Description
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany