HPLC Analysis of Benalaxyl Enantiomers on Astec CYCLOBOND™ I 2000 HP-RSP in Reversed Phase Mode
Materials
analytical column
Astec® CYCLOBOND™ I 2000 HP-RSP Chiral HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mmmobile phase component
standard
CONDITIONS
column
Astec CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)
mobile phase
[A] 10 mM ammonium acetate, pH 4.0 with acetic acid; [B] acetonitrile; (70:30, A:B)
flow rate
1 mL/min
pressure
1550 psi (107 bar)
column temp.
25 °C
detector
UV, 220 nm
injection
10 μL
sample
250 μg/mL in 75:25, water:methanol
Description
Analysis Note
Benalaxyl is a fungicide used to protect a variety of crops, including potatoes and grapes. It is a chiral compound, comprising two enantiomers. Enantioselective influences of chiral pesticides, like Benalaxyl, and their metabolites on ecosystems is an area of great importance. The possibility that only one enantiomer has the wanted properties and/or the other form an unwanted effect may exist. An analytical separation of the enantiomers is required to perform studies in these areas. The Astec CYCLOBOND™ I 2000 HP-RSP HPLC column resolves the enantiomers of Benalaxyl under reversed-phase and LC/MS-compatible conditions.
Legal Information
CYCLOBOND is a trademark of Sigma-Aldrich Co. LLC