HPLC Analysis of Benzodiazepines on Ascentis^® C8

Ascentis C8, 15 cm x 2.1 mm I.D., 3 μm particles (581402-U)

[A] 10 mM ammonium acetate (pH 6.98 unadjusted); [B] acetonitrile; (55:45, A:B)

0.2 mL/min

35 °C

MS, ESI (+), Selected Ion Recording Mode

5 μL

1 μg/mL each in 10 mM ammonium acetate (pH 6.98 unadjusted)

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany