HPLC Analysis of Beta Blockers on Ascentis^® Express C18

column

Ascentis Express C18, 5 cm × 2.1 mm I.D., 2.7 μm particles (53822-U)

mobile phase

[A] 0.1% acetic acid in water; [B] 0.1% acetic acid in acetonitrile

flow rate

0.2 mL/min

column temp.

35 °C

detector

ABI 3200 QT; ESI(+), MS/MS

injection

1 μL

instrument

Agilent 1100