HPLC Analysis of Carbidopa on Chromolith^® High Resolution RP-18e

column

Chromolith^® High Resolution RP-18 endcapped, 100 x 4.6 mm (1.52022.0001)

mobile phase

[A] methanol; [B] water + 0,1%TFA;

gradient

100% B held for 0.01 min; to 80% B in 0.9 min; held at 80% A for 9 min (Original: Initial composition: 0-0.1min 100%B, 1-10min 100%B to 80%B)

flow rate

2.5 mL/min

column temp.

ambient

detector

282 nm

injection

1 μL

sample

2,4,5-Trihydroxyphenylalanine 4μg/ml, Levodopa 402μg/ml, Methyldopa 384μg/ml, Daopamine 217μg/ml, Carbidopa 232μg/ml, 3,4-Dihydroxyphenyl acetic acid 4μg/ml, 3-o-Methylcarbidopa 4μg/ml