HPLC Analysis of Catecholamines on Discovery^® C18

column

Discovery C18, 15 cm × 4.6 mm I.D., 5 μm particles (504955)

mobile phase

[A] acetonitrile: [B] 50 mM KH₂PO₄, pH 3.0 with phosphoric acid, 100 mg/L EDTA, 200 mg/L 1-octane-sulfonic acid (5:95, A:B)

flow rate

1 mL/min

column temp.

20 °C

detector

UV, 254 nm

injection

10 μL