HPLC Analysis of Contents of Energy Drinks with SPE on Chromolith^® Performance 100 - 4.6 mm

sample preparation

1ml Sample on Extrelut N1, wait 10min., eluate with 6ml Ethylacetate, blow up Ethylacetate with nitrogen, dillute sample in 1ml water.

column

Chromolith^® Performance 100 x 4.6 mm (1.5228.0001)

mobile phase

[A] methanol; [B] 20mM Phosphat buffer pH=5; (10:90, A:B)

flow rate

0-3min 1ml/min, 3,1-12min 2,5ml/min, 12,1-15min 1ml/min

column temp.

40 °C

detector

227 nm cell: 11 μL

injection

8 μL

sample

Energy drink (Red Bull)