HPLC Analysis of Contents of Energy Drinks Without SPE on Chromolith^® Performance 100-2mm

sample preparation

direct inject

column

Chromolith^® Performance 100 x 2 mm (1.52006.0001)

mobile phase

[A] methanol; [B] 20mM Phosphat buffer pH=5; (10:90, A:B)

gradient

0-3min 190 μl/min, 3,1-12min 425μl/min, 12,1-15min 190μl/min

column temp.

40 °C

detector

227 nm cell: 1.4 μL

injection

3 μL

sample

Energy drink (Red Bull)