Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
[A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] acetonitrile; (A:B, 40:60)
1 mL/min
35 °C
UV, 234 nm
10 μL
10 μg/mL each in mobile phase
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany