HPLC Analysis of E. coli lysate with various pore size Zenix® columns
CONDITIONS
column
Zenix SEC-150, 30 cm x 7.8 mm I.D., 3 μm particles (Z777018)
column
Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)
mobile phase
150 mM Phosphate Buffer, pH 7
flow rate
1.0 mL/min
column temp.
Ambient
detector
UV, 214nm
injection
10 μL
sample
E. coli lysate, 2.5 mg/mL
Description
Analysis Note
An cell lysate, produced by destroying the membranes of E. Coli cells, is analyzed on two Zenix gel filtration columns packed with 3 micron particles. The top chromatogram shows the peak profile on a 150 Å pore size Zenix SEC-150 gel filtration column, while the bottom chromatogram shows the same sample on a 300 Å pore size Zenix SEC-300 column. As expected, the peaks shift to longer retention times on the SEC-300 column. Although roughly the same number of components are visible in each chromatogram, the SEC-150 column does a better job of pulling the peaks apart, which helps when fractions need to be collected for further analysis.
Legal Information
Zenix is a registered trademark of Sepax Technologies