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HPLC Analysis of Estrogens on Ascentis® Express Biphenyl

HPLC Analysis of Estrogens on Ascentis® Express Biphenyl application for HPLC

Materials

analytical column

Product No.
Description
Pricing

64065-U

Ascentis® Express Biphenyl Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Product No.
Description
Pricing

E1132

β-Estradiol

analytical standard

46542

α-Estradiol

VETRANAL®, analytical standard

E-074

Estriol solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

E-075

Estrone solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express Biphenyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (64065-U)

mobile phase

[A] water; [B] acetonitrile, (60:40, A:B)

flow rate

0.4 mL/min

pressure

2900 psi (200 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

2 μL

sample

60 μg/mL each in 50:50, water:organic solvent

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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