Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
methanol
1.5 mL/min
3249 psi (224 bar)
30 °C
UV, 280 nm
5 μL
0.5 mg/mL tocopherol acetate, alpha-tocopherol, delta-tocopherol and K1, 0.05 mg/mL D2, D3 and retinol acetate, 1.5 mg/mL retinol palmitate, all in ethanol.
Analysis was conducted using standard solution, detection via UV.
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany