Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
[A] water; [B] acetonitrile
25% B for 1.5 min; 25 to 85% B in 12 min; hold at 85% B for 1 min
1 mL/min
35 °C
UV, 254 nm
5 μL
50 μg/mL in 82:18, water:methanol
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany