HPLC Analysis of Hae III Digest of pBR22 DNA on TSKgel® NPR, High Resolution, Fast Analysis
Materials
related product
Product No.
Description
Pricing
TSKgel® DEAE-NPR HPLC Column
phase DEAE (diethylaminoethyl), L × I.D. 3.5 cm × 4.6 mm, 2.5 μm particle sizeused together
Product No.
Description
Pricing
TSKgel® DNA-NPR Guard Column
phase DEAE (diethylaminoethyl), L × I.D. 0.5 cm × 4.6 mm, 5 μm particle sizeCONDITIONS
column
TSKgel DEAE-NPR, 3.5 cm x 4.6 mm I.D., 2.5 μm particles (813075)
column
TSKgel DEAE-NPR gaurd column, 0.5 cm x 4.6 mm I.D., 5 μm particles (817088)
mobile phase
[A] 0.02 M Tris-HCl, pH 9.0; [B] 0.02 M Tris-HCl, 1.0 M NaCl, pH 9.0
gradient
48 to 65% B in 15 min
flow rate
1.5 mL/min
pressure
2000 psi 138 bar
column temp.
40 °C
detector
UV, 260 nm
sample
Hae III Digest of pBR22 DNA, base pair number for each peak is indicated on the figure
Description
Other Notes
Data provided by Tosoh Bioscience LLC.
Legal Information
TSKgel is a registered trademark of Tosoh Corporation