HPLC Analysis of Hippuric and Methylhippuric Acids in Urine on SUPELCOSIL™ LC-18
Materials
related product
Product No.
Description
Pricing
CONDITIONS
column
SUPELCOSIL LC-18, 7.5 cm × 4.6 mm I.D., 3 μm particles (58984)
mobile phase
[A] tetrahydrofuran:10 mM potassium phosphate, pH 3.0 [B] with phosphoric acid (3:97, A:B)
flow rate
1.5 mL/min
detector
UV, 260 nm
injection
10 μL spiked urine (300 μg/mL HA, o-MHA; 150 μg/mL m-MHA, p-MHA)
Description
Analysis Note
Urinary excretion of hippuric acid and m-or p-methylhippuric acid in the urine is a test of exposure to toluene and m-or p-xylene vapors.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC