HPLC Analysis of Iloperidone and Hydroxy Iloperidone Using a Titan C18 Column
Materials
used together
CONDITIONS
column
Titan C18, 10 cm x 3.0 mm I.D., 1.9 μm particles (577160-U)
mobile phase
[A] 10 mM ammonium formate; pH 3.0 with formic acid [B] 0.1% formic acid in methanol
gradient
45% B for 2 minutes followed by a linear gradient to 80% B for 10 minutes, followed by a hold at 80% B for 2 minutes.
flow rate
0.5 mL/min
pressure
6500 psi (445 bar)
column temp.
35 °C
detector
UV, 275 nm
injection
2 μL
sample
50 μg/mL and 5 μg/mL of hydroxy metabolite in 10% methanol
Description
Analysis Note
Iloperidone is an atypical antipsychotic administered for the treatment of schizophrenia. The drug is marketed under the trade names Fanapt® and Fanapta. Hydroxy iloperidone is a major metabolite of the atypical antipsychotic iloperidone which is administered for the treatment of schizophrenia. The metabolite is also pharmacologically active, possessing central nervous system (CNS) activity.
Legal Information
Fanapt is a registered trademark of Vanda Pharmaceuticals
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