HPLC Analysis of Insulin on SRT® SEC-150
Materials
related product
Product No.
Description
Pricing
Zenix® SEC-150 Gel Filtration Column, 3 μm
L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steelused together
Product No.
Description
Pricing
Zenix® SEC-150 Gel Filtration Guard Column, 3 μm
L × I.D. 5 cm × 7.8 mm, 3 μm particle size, stainless steelCONDITIONS
column
SRT SEC-150, 30 cm x 7.8 mm I.D., 5 μm particles, 150 Å (Z777018)
mobile phase
0.1% Arginine: ACN: Acetic Acid = 65:20:15 (V/V/V)
flow rate
0.5 mL/min
column temp.
Ambient
detector
UV, 214 nm
injection
100 μL
sample
4.01 mg/mL Insulin
Description
Analysis Note
The analysis of insulin by gel filtration chromatography is shown on a SRT SEC-150 column. The composition of the mobile phase, 20% acetonitrile in the presence of acetic acid and arginine, indicates that insulin is a very hydrophobic peptide. At higher sensitivity the chromatogram shows several degradation products or contaminants eluting before the insulin peak, a position where one would expect compounds of higher molecular mass to elute.
Legal Information
SRT is a registered trademark of Sepax Technologies