HPLC Analysis of Insulin Variants and Analogs on BIOshell A160 Peptide CN
Materials
related product
CONDITIONS
column
BIOshell A160 Peptide CN, 15 cm x 2.1 mm, 2.7 μm particles (66969-U)
mobile phase
[A] 75:25 water (0.1% trifluoroacetic acid): acetonitrile (0.1% trifluoroacetic acid); [B] 50:50 water (0.1% trifluoroacetic acid): acetonitrile (0.1% trifluoroacetic acid); (87:13, A:B)
flow rate
0.5 mL/min
pressure
3200 psi (220 bar)
column temp.
75 °C
detector
UV, 215 nm
injection
0.5 μL
sample
Mixture of six insulin variants, 100 μg/mL in 0.1% aqueous trifluoroacetic acid
Description
Analysis Note
The BIOshell A160 Peptide CN, with cyanopropyl functional groups, can provide alternative selectivity to a traditional C18 phase. Selectivity using the CN phase is primarily due to hydrophobicity and dipole-dipole interactions. A mixture of six insulin variants was separated using the BIOshell A160 Peptide CN column. Alternative selectivity of the first two analytes is seen using this column compared to the BIOshell A160 Peptide C18.
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