HPLC Analysis of Isocyanates on Titan^™ C18

column

Titan C18, 5 cm x 2.1 mm I.D., 1.9 μm particles (577122-U)

mobile phase

[A] 0.05% formic acid in 5:95 acetonitrile:water; [B] 0.05% formic acid in 95:5 acetonitrile:water

gradient

50% to 90% B in 1 min, hold at 90% B for 3 min, 90% to 50% B in 0.1 min, hold at 50% B for 3.9 min

flow rate

0.5 mL/min

pressure

3916 psi (270 bar)

column temp.

30 °C

detector

MS, ESI (+)

detector

MRM

injection

2 μL

sample

isocyanates in acetonitrile, 0.06 μg/mL