HPLC Analysis of L-Cysteine on SeQuant^® ZIC-HILIC

column

SeQuant ZIC-HILIC, PEEK, 100 x 4.6 mm, 5 μm, 200 Å (1.50453.0001)

mobile phase

[A] acetonitrile; [B] ammonium acetate; (80:20, A:B)

flow rate

0.5 mL/min

column temp.

ambient

detector

220 nm

injection

1 μL

sample

1 mg/mL of acetylcysteine in acetonitrile/water (70/30)