HPLC Analysis of mAb on Antibodix^® WCX, Effect of Particle Size (5 cm columns)

column

Antibodix WCX-NP3 PEEK, 5 cm x 4.6 mm I.D., 3 μm particles (Z777288)

column

Z777280 (Antibodix WCX-NP1.7, 5 cm x 4.6 mm, 1.7 μm particles)

mobile phase

(A) 10 mM sodium phosphate buffer, pH 7.5; (B) A + 100 mM NaCl

gradient

NP3 column: 15 to 55% B in 30 min, held for 3 min;

NP1.7 column: 15 to 100% B in 60 min, held for 1.7 min

flow rate

0.5 mL/min for 3 min; 0.3 mL/min for 1.7 min

pressure

2180 psi (3 min), 3425 psi (1.7 min)

column temp.

ambient

detector

UV, 214 nm

injection

5 μL

sample

Monoclonal antibody, 5 mg/mL