HPLC Analysis of Medium MW Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size
Materials
related product
Product No.
Description
Pricing
Zenix® SEC-100 Gel Filtration Column, 3μm
L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steelCONDITIONS
column
Zenix SEC-100, 30 cm x 4.6 mm I.D., 3 μm particles (Z777006)
column
Zenix SEC-150, 30 cm x 4.6 mm I.D., 3 μm particles (Z777016)
column
Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)
mobile phase
0.2 M sodium phosphate dibasic, pH 7.0 with phosphoric acid
flow rate
0.25 mL/min
pressure
1000 psi (69 bar)
column temp.
25 °C
detector
UV, 215 nm
injection
0.5 μL
sample
Waters BEH200 protein standard
Description
General description
Pore size is a kay parameter of resolution size exclusion chromatography. Matching the pore size to resolution of the sample components is critical in selecting a column. This application shows the impact of pore size on resolution of sample components as a function of molecular size
Legal Information
Zenix is a registered trademark of Sepax Technologies