HPLC Analysis of Metformin and Related Impurities Cyanoguanidine and Melamine on SeQuant® ZIC-HILIC
CONDITIONS
column
Sequant ZIC-HILIC, PEEK, 250 x 4.6 mm, 5 μm, 200 Å (1.50458.0001)
mobile phase
[A] 10 mM ammonium acetate; [B] acetonitrile; (15:85, A:B)
flow rate
1.5 mL/min
pressure
913 psi (63 bar)
column temp.
30 °C ambient
detector
218 nm (cell: 10 μL)
sample
500 ppm metformin and 4 ppm of each impurity in mobile phase
Test paramet
ersTest parameters500 ppm metformin and 4 ppm of each impurity in mobil
injection
10 μL
Description
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany