HPLC Analysis of Methcathinone and Ephedrine on Discovery^® HS F5

column

Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567516-U)

mobile phase

[A] 10 mM ammonium acetate (pH 6.98); [B] acetonitrile (30:70, A:B)

flow rate

2 mL/min

column temp.

35 °C

detector

photodiode array

injection

5 μL