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HPLC Analysis of Mouse Ascites Fluid on TSKgel® BioAssist® Q vs. Competitive Q Column

HPLC Analysis of Mouse Ascites Fluid on TSKgel® BioAssist® Q vs. Competitive Q Column application for HPLC

Materials

related product

Product No.
Description
Pricing

TSKgel® BioAssist® Q HPLC Column

phase polyamine, L × I.D. 5 cm × 4.6 mm, 10 μm particle size, PEEK hardware

TSKgel® BioAssist® Q HPLC Column

phase polyamine, L × I.D. 10 cm × 10 mm, 13 μm particle size, PEEK hardware

used together

Product No.
Description
Pricing

Sodium chloride

Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Tris hydrochloride

>99% (titration), pH 7.0-9.0, suitable for FISH

CONDITIONS

column

TSKgel BioAssist Q, 5 cm x 4.6 mm I.D., 10 μm particles (819685)

gradient

15 min linear gradient of NaCl from 0 to 1 M in 20 mM Tris-HCl buffer, pH 8.0

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 280 nm

injection

5 μL

sample

mouse ascites fluid (3-fold dilution in 20 mM Tris-HCl buffer, pH 8.0)

Description

Other Notes

Data provided by Tosoh Bioscience LLC.

Legal Information

BioAssist is a registered trademark of Tosoh Corporation, TSKgel is a registered trademark of Tosoh Corporation