BIOshell Glycan HPLC
Column (50993-U)
(A) Water with 100 mM ammonium formate, pH 4.5; (B) Acetonitrile
min, %B, Flow (mL/min): 0-74-0.4; 15-71-0.4; 25-55-0.4; 26-45-0.2; 30-45-0.2; 31-75-0.4; 35-75-0.4.
1130 psi (78 bar)
50 °C
Fluorescence (λex = 260 nm, λem = 430 nm)
3 μL
in 70% mobile phase B
Separation pf a mix of 2-AB labled N-linked glycans released from human IgG was optimized on BIOshell Glycan HPLC Columns. Baseline resolutions for all of the individual glycans were observed in both mixes, except for the positional isomer pair G1F and G1F′ which were partially resolved.
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