HPLC Analysis of Nateglinide Tablet on Purospher® STAR RP-18 endcapped
CONDITIONS
sample preparation
Take 10 tablets of Nagelienai Pian, weigh precisely, grind to powder. Weight appropriate amount (roughly contained 70mg Nateglinide ) to a 100-mL volumetric flask, (add appropriate amount of 55% ACN solution. Shake for 30 minutes. Add diluent to volume. Filtrate.)
column
Purospher STAR RP-18 endcapped 150 x 4.6 mm (1.51455.0001)
mobile phase
[A] 0.05% trifluoroacetic acid solution; [B] acetonitrile; (40:60, A:B)
flow rate
1 mL/min
pressure
1160 psi (80 bar)
column temp.
30 °C
detector
210 nm (cell: 1 μL/10 mm)
injection
10 μL
sample
30 μg/mL (30 ppm) of Nateglinide in diluent to obtain the standard solution.
Description
Legal Information
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany