HPLC Analysis of Organic Acids in Riesling Wine on SeQuant^® ZIC-HILIC

column

SeQuant ZIC-HILIC (5 μm, 200 Å) PEEK, 150x2.1 mm (1.50454.0001)

mobile phase

[A] acetonitrile; [B] potassium phosphate; (75:25, A:B)

flow rate

0.4 mL/min

column temp.

30 °C

detector

200 nm

detector

Shimadzu LC-10Vp equipped with 2.5μL semi-micro flow-cell

injection

5 μL

sample

Riesling wine or 10 ppm mix of standards