HPLC Analysis of Organic Acids on Chromolith® HighResolution RP-18 endcapped

Materials
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CONDITIONS
column
Chromolith HighResolution RP-18, 10 cm x 4.6 mm I.D. (1.52022) (1.52022.0001)
mobile phase
10 mM diammonium hydrogen phosphate, pH 2.7
flow rate
1.0 mL/min
pressure
464 psi 32 bar
column temp.
30 °C
detector
UV, 210 nm
injection
20 μL
sample
tartaric acid, malic acid, citric acid, each 1.0 mg/mL in water, succinic acid, 0.2 mg/mL in water
Description
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany