HPLC Analysis of Pepides, Alberta Mix, on Discovery^® BIO Wide Pore C18, No TFA

column

Discovery BIO Wide Pore C18, 15 cm × 2.1 mm I.D., 3 μm particles (567202-U)

mobile phase

[A] 25 mM formic acid in water, [B] (25 mM formic acid in water):(20 mM formic acid in acetonitrile, (50:50)

flow rate

0.20 mL/min

column temp.

ambient

detector

+ES

injection

1 μL