HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (5 cm Column)
CONDITIONS
column
Discovery BIO Wide Pore C18, 5 cm × 4.6 mm I.D., 5 μm particles (568220-U)
mobile phase
[A] (5:95), 0.5% TFA in water:0.5% TFA in acetonitrile; [B] (25:75), 0.5% TFA in water:0.5% TFA in acetonitrile
gradient
2-24% B in 22 min, 8 min. at 100%A
flow rate
2 mL/min
column temp.
70 °C
detector
UV, 220 nm
Description
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany